Active Recombinant Human VSTM2B protein, Fc-Avi-tag-tagged, Biotinylated

• Cat.No.: VSTM2B-051H
• Product Overview: Biotinylated Recombinant Human VSTM2B(Ala29-Thr263) protein, fused to Fc-Avi-tag tag at the C-terminus, was expressed in CHO cells .

Specification

• Species: Human
• Source: CHO
• Tag: Avi&Fc
• Protein Length: Ala29-Thr263
• Description: V-set and transmembrane domain-containing protein 2B (VSTM2B) is a type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily. There have been 8 VSTM family members identified and several have been shown to exhibit immunosuppressive activity (1). Human VSTM2B consists of an extracellular domain (ECD) with an Ig-like V-type domain, a transmembrane domain, and single amino acid (aa) in the cytoplasm. The mature ECD of human VSTM2B shares 86% and 85% aa sequence identity with mouse and rat VSTM2B, respectively. Currently, both the expression pattern and biological functions of VSTM2B remain unknown. Our in-house data show that VSTM2B inhibits the human T cell activation, including anti-CD3 induced IL-2, IFN-gamma secretion, and T cell proliferation. Our Avi-tag Biotinylated Recombinant VSTM2B features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
• Predicted N Terminal: Ala29
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA.
• Molecular Mass: 60-72 kDa, under reducing conditions
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: V-set and transmembrane domain containing 2B[ Homo sapiens ]
• Official Symbol: VSTM2B
• Gene ID: 342865
• mRNA Refseq: NM_001146339.1
• Protein Refseq: NP_001139811.1
• UniProt ID: A6NLU5