Active Recombinant Human VNN1 protein, His-tagged

• Cat.No.: VNN1-3229H
• Product Overview: Recombinant Human VNN1 protein(NP_004657.2)(Met1-Ser490), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ser490
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to hydrolyze pantetheine to pantothenate and cysteamine. The specific activity is >3000 pmol/min/μg.
• Molecular Mass: The recombinant human VNN1 consists of 480 amino acids and has a predicted molecular mass of 53.7 kDa. It migrates as an approximately 70-75 kDa band in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VNN1 vanin 1 [ Homo sapiens ]
• Official Symbol: VNN1
• Synonyms: VNN1; vanin 1; pantetheinase; Tiff66; vanin-1; vannin 1; pantetheine hydrolase; vascular non-inflammatory molecule 1; HDLCQ8; MGC116930; MGC116931; MGC116932; MGC116933
• Gene ID: 8876
• mRNA Refseq: NM_004666
• Protein Refseq: NP_004657
• UniProt ID: O95497