Active Recombinant Human VEGFA Protein, None-tagged, Alexa Fluor 647 conjugated

• Cat.No.: VEGFA-210HAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant human VEGFA (P15692-4) (Met 1-Arg 191) was produced in Baculovirus-Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Protein Length: Met1-Arg209
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 50-210 ng/mL.
• Molecular Mass: The recombinant human VEGF 183 isoform consists of 183 amino acids and has a predicted molecular mass of 21 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF; vascular permeability factor; VEGF; MVCD1; MGC70609
• Gene ID: 7422
• mRNA Refseq: NM_001025366
• Protein Refseq: NP_001020537
• UniProt ID: P15692