Active Recombinant Human USP15 Protein, His-tagged

• Cat.No.: USP15-358H
• Product Overview: Recombinant Human USP15 Protein is produced by Spodoptera frugiperda, Sf 21 (baculovirus) expression system. This protein is fused with a His tag at the C-terminal.

Specification

• Description: Ubiquitin carboxyl-terminal hydrolase 15 (USP15) is a specialized cysteine protease with a predicted molecular weight of 112 kDa. USP15 is a member of the peptidase C19 family and the human protein shares 98% amino acid sequence identity with its mouse ortholog. USP15 is a regulator of the TGF beta signaling pathway, where it may promote signaling strength by deubiquitination of SMAD proteins and/or the TGF beta R1. Other reported targets of USP15 include histone H2B, NF kappa BI alpha, MDM2 and human papillomavirus type 16 E6 protein.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: X mg/ml (XμM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP, 10% (v/v) Glycerol
• Bio-activity: Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP15 concentration of 5-20 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates.
• Molecular Mass: 113 kDa
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: USP15 ubiquitin specific peptidase 15 [ Homo sapiens ]
• Official Symbol: USP15
• Synonyms: USP15; KIAA0529; UNPH4; UNPH-2; FLJ55073; MGC74854; MGC131982; MGC149838
• Gene ID: 9958
• mRNA Refseq: NM_001252078
• Protein Refseq: NP_001239007
• MIM: 604731
• UniProt ID: Q9Y4E8