Active Recombinant Human UBE2C Protein, Met & His-tagged

• Cat.No.: UBE2C-899H
• Product Overview: Recombinant Human UBE2C (Accession # O00762), fused with an N-terminal Met and 7-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: X mg/ml (X µM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
• Bio-activity: Recombinant Human His6-UbcH10/UBE2C is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UbcH10/UBE2C concentration of 0.1-1 µM.
• Molecular Mass: Recombinant Human UBE2C, Met & His-tagged has a calculated MW of 21 kDa.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: UBE2C ubiquitin-conjugating enzyme E2C [ Homo sapiens ]
• Official Symbol: UBE2C
• Synonyms: UBE2C; ubiquitin-conjugating enzyme E2C; ubiquitin-conjugating enzyme E2 C; UBCH10; ubiquitin-protein ligase C; ubiquitin carrier protein C; ubiquitin carrier protein E2-C; cyclin-selective ubiquitin carrier protein; mitotic-specific ubiquitin-conjugating enzyme; dJ447F3.2
• Gene ID: 11065
• mRNA Refseq: NM_007019
• Protein Refseq: NP_008950
• MIM: 605574
• UniProt ID: O00762
• Chromosome Location: 20q13.12