Active Recombinant Human UBE2A Protein, His-tagged
Cat.No. : | UBE2A-530H |
Product Overview : | Recombinant Human UBE2A (Accession # NP_003327), fused with an N-terminal 6-His tag, was produced in E. coli. |
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Species : | Human |
Source : | E.coli |
Tag : | His |
Form : | X mg/ml (XµM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP |
Bio-activity : | Recombinant Human His6-HR6A/UBE2A is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-HR6A/UBE2A concentration of 0.1-1 µM. |
Molecular Mass : | Recombinant Human UBE2A, His-tagged has a calculated MW of 19 kDa. |
Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. |
Storage : | Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Reconstitution : | Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Gene Name | UBE2A ubiquitin-conjugating enzyme E2A [ Homo sapiens ] |
Official Symbol | UBE2A |
Synonyms | UBE2A; ubiquitin-conjugating enzyme E2A; ubiquitin conjugating enzyme E2A (RAD6 homolog); ubiquitin-conjugating enzyme E2 A; HHR6A; RAD6A; UBC2; HR6A; RAD6 homolog A; ubiquitin-protein ligase A; ubiquitin carrier protein A; ubiquitin-conjugating enzyme E2A (RAD6 homolog); MRXSN; MRXS30; |
Gene ID | 7319 |
mRNA Refseq | NM_003336 |
Protein Refseq | NP_003327 |
MIM | 312180 |
UniProt ID | P49459 |
Chromosome Location | Xq24 |
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UBE2A-4866R | Recombinant Rhesus Macaque UBE2A Protein, His (Fc)-Avi-tagged | +Inquiry |
UBE2A-530H | Active Recombinant Human UBE2A Protein, His-tagged | +Inquiry |
◆ Cell & Tissue Lysates | ||
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Not For Human Consumption!
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