Active Recombinant Human TNFSF13B Protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: TNFSF13B-425H
• Product Overview: Biotinylated Recombinant Human TNFSF13B Protein (Ala 134 - Leu 285) (AAH20674.1), fused with an Avi tag at the N-terminus, followed by a human IgG1 Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: Ala 134 - Leu 285
• Predicted N Terminal: Ala 134
• Form: Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human BCMA, Fc Tag at 5μg/mL (100 μL/well) can bind Biotinylated Human BAFF, Fc Tag with a linear range of 1.95-15.6 ng/mL.
• Molecular Mass: Recombinant Human TNFSF13B Protein has a calculated MW of 44.9 kDa. As a result of Glycosylation, The protein migrates as 50 kDa under reducing (R) condition and 200 kDa under non-reducing (NR) condition on SDS-PAGE gel.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF13B tumor necrosis factor (ligand) superfamily, member 13b [ Homo sapiens ]
• Official Symbol: TNFSF13B
• Synonyms: TNFSF13B; tumor necrosis factor (ligand) superfamily, member 13b; TNFSF20; tumor necrosis factor ligand superfamily member 13B; BAFF; BLYS; CD257; TALL 1; TALL1; THANK; delta BAFF; Delta4 BAFF; B-lymphocyte stimulator; B-cell-activating factor; ApoL related ligand TALL-1; TNF homolog that activates apoptosis; dendritic cell-derived TNF-like molecule; tumor necrosis factor-like protein ZTNF4; TNF and ApoL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily, member 20; DTL; ZTNF4; TALL-1
• Gene ID: 10673
• mRNA Refseq: NM_001145645
• Protein Refseq: NP_001139117
• MIM: 603969
• UniProt ID: Q9Y275
• Chromosome Location: 13q32-q34
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Intestinal immune network for IgA production, organism-specific biosystem; Intestinal immune network for IgA production, conserved biosystem; Rheumatoid arthritis, organism-specific biosystem; Rheumatoid arthritis, conserved biosystem
• Function: cytokine activity; protein binding; receptor binding; tumor necrosis factor receptor binding