Active Recombinant Human TNFSF13B Protein (Ala134-Leu285), C-His tagged, Animal-free, Carrier-free

• Cat.No.: TNFSF13B-09H
• Product Overview: Recombinant Human TNFSF13B Protein (Ala134-Leu285) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: BAFF also known as BLYS, TALL-1 and TNFSF13B, which belongs to tumor necrosis factor family. BAFF is a 31.2 kDa type II transmembrane protein containing 285 residues that predominantly produced by myeloid cells. BAFF has been demonstrated to activate the survival of B-cells and the B-cell response by binding to BAFFR/BR3. Additionally, BAFF also takes part in regulating B and T cell function via forming two ligands-two receptors pathway through sharing TNFRSF13B/TACI and TNFRSF17/BCMA receptors with APRIL.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is < 0.5 ng/mL.
• Molecular Mass: The protein has a calculated MW of 17.98 kDa.
  The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Ala134-Leu285
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: TNFSF13B tumor necrosis factor (ligand) superfamily, member 13b [ Homo sapiens (human) ]
• Official Symbol: TNFSF13B
• Synonyms: TNFSF13B; tumor necrosis factor (ligand) superfamily, member 13b; TNFSF20; tumor necrosis factor ligand superfamily member 13B; BAFF; BLYS; CD257; TALL 1; TALL1; THANK; delta BAFF; Delta4 BAFF; B-lymphocyte stimulator; B-cell-activating factor; ApoL related ligand TALL-1; TNF homolog that activates apoptosis; dendritic cell-derived TNF-like molecule; tumor necrosis factor-like protein ZTNF4; TNF and ApoL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily, member 20; DTL; ZTNF4; TALL-1
• Gene ID: 10673
• mRNA Refseq: NM_001145645
• Protein Refseq: NP_001139117
• MIM: 603969
• UniProt ID: Q9Y275