Active Recombinant Human TNFSF11 protein, rFc-tagged(HPLC-verified)

• Cat.No.: TNFSF11-425H
• Product Overview: Recombinant Human TNFSF11 protein (AAC51762.1) (Gly64-Asp245), fused with rFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: rFc
• Protein Length: Gly64-Asp245
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1.Immobilized Recombinant Human Osteoprotegerin / TNFRSF11B Protein (His Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human RANKL Protein (ECD, rFc Tag), the EC50 is 15-45 ng/mL.
  2.The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is typically 1.5-7.5 ng/mL.
• Molecular Mass: The recombinant human TNFSF11 comprises 410 amino acids and has a predicted molecular mass of 46.18 kDa. The apparent molecular mass of the protein is approximately 134.21 kDa and 54.28 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF11 tumor necrosis factor (ligand) superfamily, member 11 [ Homo sapiens ]
• Official Symbol: TNFSF11
• Synonyms: TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11; CD254; ODF; OPGL; RANKL; TRANCE; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; sOdf; OPTB2; hRANKL2
• Gene ID: 8600
• mRNA Refseq: NM_003701
• Protein Refseq: NP_003692
• MIM: 602642
• UniProt ID: O14788