Active Recombinant Human TNFSF11

• Cat.No.: TNFSF11-251H
• Product Overview: Recombinant Human TNFSF11 (Accession # AAC51762) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Non
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in MOPS and NaCl with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells.The ED50 for this effect is typically 1.5-7.5 ng/mL in the presence of 2.5 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.
• Molecular Mass: Recombinant Human TNFSF11 has a calculated MW of 23 kDa. In SDS-PAGE migrates as 35 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: TNFSF11 tumor necrosis factor (ligand) superfamily, member 11 [ Homo sapiens ]
• Official Symbol: TNFSF11
• Synonyms: TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11; CD254; ODF; OPGL; RANKL; TRANCE; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; sOdf; OPTB2; hRANKL2
• Gene ID: 8600
• mRNA Refseq: NM_003701
• Protein Refseq: NP_003692
• MIM: 602642
• UniProt ID: O14788
• Chromosome Location: 13q14