Active Recombinant Human TNFRSF8 Protein, Fc-tagged, FITC conjugated

• Cat.No.: TNFRSF8-1594HF
• Product Overview: FITC conjugated recombinant human CD30 (amino acid residues 1 – 379) encoding the extracellular domain was fused to the carboxy-terminal polyhistidine tagged Fc region of human IgG1 via a polypeptide linker. The chimeric protein was expressed inNSO cells.

Specification

• Description: CD30/TNFRSF8, a type I transmembrane glycoprotein belonging to the TNF receptor superfamily and tumor marker. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. It is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. CD30 is associated with anaplastic large cell lymphoma. It is expressed in embryonal carcinoma but not in seminoma and is thus is a useful marker in distinguishing between these germ cell tumors.
• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Bio-activity: The biological activity of Human CD30 was determined by its ability to bind rhCD30 Ligand. Immobilized rhCD30 Ligand at 100 ng/mL (100 μL/well) can bind rhCD30/Fc with a linear range of 3.125-200 ng/mL in a functional ELISA.
• Molecular Mass: 100-125 kDa
• Endotoxin: < 1.0 EU/ μg as determined by the LAL method
• Purity: > 95 % by SDS-PAGE and analyzed by silver stain.
• Characteristic: Disulfide-linked homodimer
  Labeled with FITC via amines
  Excitation source: 488 nm spectral line, argon-ion laser
  Excitation Wavelength: 488 nm
  Emission Wavelength: 535 nm
• Stability: This lyophilized protein is stable for 6 to 12 months when stored desiccated at -20 to -70 centigrade. After aseptic reconstitution, this protein may be stored at 2 to 8 centigrade for one month or at -20 to -70 centigrade in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
• Storage: This lyophilized protein is stable for six to twelve months when stored desiccated at -20 to -70 centigrade. After aseptic reconstitution, this protein may be stored at 2 to 8 centigrade for one month or at -20 to -70 centigrade in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
• Storage Buffer: This recombinant protein was 0.2 μm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2-7.3 with no calcium, magnesium, or preservatives.
• Protein length: 1-379 a.a.

Gene Information

• Gene Name: TNFRSF8 tumor necrosis factor receptor superfamily, member 8 [ Homo sapiens ]
• Official Symbol: TNFRSF8
• Synonyms: TNFRSF8; tumor necrosis factor receptor superfamily, member 8; CD30; Ki-1; D1S166E; Tumor necrosis factor receptor superfamily member 8; CD30L receptor; Lymphocyte activation antigen CD30; Ki-1 antigen; OTTHUMP00000001783; CD30 antigen
• Gene ID: 943
• mRNA Refseq: NM_001243
• Protein Refseq: NP_001234
• MIM: 153243
• UniProt ID: P28908