Active Recombinant Human TNFRSF13B Protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: TNFRSF13B-694H
• Product Overview: Biotinylated Human TACI, Fc Tag, Avi Tag is expressed from HEK293 cells. It contains AA Ser 2 - Thr 166 (Accession # O14836-1). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 2-166 a.a.
• Predicted N Terminal: Ser 2
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Human BAFF, Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human TACI, Fc Tag, Avi Tag with a linear range of 1.2-39 ug/mL.
• Molecular Mass: The protein has a calculated MW of 46.8 kDa. The protein migrates as 33-40 kDa and 47-50 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: TNFRSF13B tumor necrosis factor receptor superfamily, member 13B [ Homo sapiens ]
• Official Symbol: TNFRSF13B
• Synonyms: TNFRSF13B; tumor necrosis factor receptor superfamily, member 13B; tumor necrosis factor receptor superfamily member 13B; CD267; TACI; tumor necrosis factor receptor 13B; transmembrane activator and CAML interactor; CVID; CVID2; TNFRSF14B; FLJ39942; MGC39952; MGC133214
• Gene ID: 23495
• mRNA Refseq: NM_012452
• Protein Refseq: NP_036584
• MIM: 604907
• UniProt ID: O14836