Active Recombinant Human TNFRSF12A, Fc-tagged, Biotinylated

• Cat.No.: TNFRSF12A-709H
• Product Overview: The recombinant human TWEAKR-Fc fusion is expressed as a 281 amino acid protein consisting of Glu28- Pro80 region of TWEAKR (UniProt accession #Q9NP84) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

Specification

• Species: Human
• Source: Human Cells
• Tag: Fc
• Form: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule).
• Bio-activity: Binds to its ligand TWEAK/TNFSF12 and inhibits TWEAK-mediated signaling activities including TWEAK--induced apoptosis in HT-29 human colon cancer cells and proliferation of human umbilical vein endothelial cells (HUVECs).
• Molecular Mass: Calculated molecular mass (kDa): 31.1; Estimated by SDS-PAGE under reducing condition (kDa): ~40
• Endotoxin: <0.1 eu="" per="" 1="" μg="" of="" purified="" recombinant="" protein="" determined="" by="" the="" lal="">
• Purity: >95% judged by SDS-PAGE under reducing condition.
• Storage: The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: TNFRSF12A tumor necrosis factor receptor superfamily, member 12A [ Homo sapiens ]
• Official Symbol: TNFRSF12A
• Synonyms: TNFRSF12A; tumor necrosis factor receptor superfamily, member 12A; tumor necrosis factor receptor superfamily member 12A; CD266; FN14; TweakR; tweak-receptor; FGF-inducible 14; type I transmembrane protein Fn14; fibroblast growth factor-inducible immediate-early response protein 14; TWEAKR
• Gene ID: 51330
• mRNA Refseq: NM_016639
• Protein Refseq: NP_057723
• MIM: 605914
• UniProt ID: Q9NP84
• Chromosome Location: 16p13.3
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem
• Function: protein binding; receptor activity