Active Recombinant Human TNF Protein, Animal Free

• Cat.No.: TNF-49H
• Product Overview: Recombinant Human TNF Protein without tag was expressed in Human cells.
  Animal Free and Xeno Free

Specification

• Species: Human
• Source: Human Cells
• Description: Tumor necrosis factor alpha (TNF-α) is an EGF-related polypeptide growth factor that signals through the TNFRSF1A/TNFR1 and TNFRSF1B/ TNFBR. It is mainly secreted by macrophages and also produced by monocytes, keratinocytes, and various tumor cells. TNF-α stimulates the proliferation of a wide range of epidermal and epithelial cells. TNF-α is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. Under certain conditions it can stimulate cell proliferation and induce cell differentiation, impair regulatory T cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation.
• Bio-activity: The activity was measured by the cytotoxicity in L-929 cell in the presence of actinomycin D.
• Molecular Mass: 17 kDa
• Endotoxin: <0.5 EU/ μg
• Purity: High Purity, >95% by SDS-PAGE
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -80 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS without carrier protein
• Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Gene Information

• Gene Name: TNF tumor necrosis factor [ Homo sapiens (human) ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; TNFA, tumor necrosis factor (TNF superfamily, member 2); DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; TNFA; TNF-alpha
• Gene ID: 7124
• mRNA Refseq: NM_000594
• Protein Refseq: NP_000585
• MIM: 191160
• UniProt ID: P01375