Active Recombinant Human TLR2 Protein, His-tagged

• Cat.No.: TLR2-529H
• Product Overview: Recombinant Human TLR2 (Accession # NP_003255) Glu21-Leu590, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Glu21-Leu590
• Predicted N Terminal: Glu21
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to bind biotinylated Pam3CysSerLys4 (PAM3CSK4) (2 µg/mL). The 50% maximum binding is achieved at 0.1-0.5 µg/mL of Recombinant Human TLR2. Results can vary with different preparations of biotinylated PAM3CSK4.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human TLR2, His-tagged has a calculated MW of 65.7 kDa. In SDS-PAGE migrates as 75-80 kDa, reducing conditions.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: TLR2 toll-like receptor 2 [ Homo sapiens ]
• Official Symbol: TLR2
• Synonyms: TLR2; toll-like receptor 2; CD282; TIL4; toll/interleukin 1 receptor-like 4; toll/interleukin-1 receptor-like protein 4
• Gene ID: 7097
• mRNA Refseq: NM_003264
• Protein Refseq: NP_003255
• MIM: 603028
• UniProt ID: O60603
• Chromosome Location: 4q32