Active Recombinant Human TGM4, Met&His-tagged

• Cat.No.: TGM4-245H
• Product Overview: Recombinant Human TGM4 (Accession # AAC50516) Met2-Lys684, fused with an N-terminal Met and 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf 21 Insect cells
• Species: Human
• Tag: His
• Predicted N Terminal: No results obtained, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments.
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, DTT and Glycerol.
• Bio-activity: Measured by its ability to form CBZ-Gln-Gly-Hydroxamate from CBZ-Gln-Gly and Hydroxylamine.The specific activity is >50 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human TGM4, Met&His-tagged has a calculated MW of 78 kDa. In SDS-PAGE migrates as 65-70 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Protein length: 2-684 a.a.

Gene Information

• Gene Name: TGM4 transglutaminase 4 (prostate) [ Homo sapiens ]
• Official Symbol: TGM4
• Synonyms: TGM4; transglutaminase 4 (prostate); protein-glutamine gamma-glutamyltransferase 4; TGP; TG(P); TGase P; TGase-4; fibrinoligase; transglutaminase P; transglutaminase-4; prostate transglutaminase; prostate-specific transglutaminase; hTGP; FLJ26776
• Gene ID: 7047
• mRNA Refseq: NM_003241
• Protein Refseq: NP_003232
• MIM: 600585
• UniProt ID: P49221
• Chromosome Location: 3p22-p21.33