Active Recombinant Human TCN2 protein(Met1-Trp427), His-tagged

• Cat.No.: TCN2-873H
• Product Overview: Recombinant Human TCN2 (NP_000346.2) (Met 1-Trp 427) with a C-terminal polyhistidine tag was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Trp427
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized CD320 Protein, Mouse, Recombinant (hFc Tag) at 2 μg/mL (100 μL/well) can bind TCN2 Protein, Human, Recombinant (His Tag), the EC50 is 10-62 ng/mL.
• Molecular Mass: The secreted recombinant human TCN2 comprises 418 amino acids with a predicted molecular mass of 46.7 kDa. It migrates with the molecular weight of approximately 43 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TCN2 transcobalamin II [ Homo sapiens ]
• Official Symbol: TCN2
• Synonyms: TCN2; transcobalamin II; transcobalamin II; macrocytic anemia; transcobalamin-2; D22S676; D22S750; macrocytic anemia; TC2; vitamin B12-binding protein 2; II; TC; TC-2; TCII; TC II
• Gene ID: 6948
• mRNA Refseq: NM_000355
• Protein Refseq: NP_000346
• MIM: 613441
• UniProt ID: P20062