Active Recombinant Human SUMO2, His-tagged

• Cat.No.: SUMO2-588H
• Product Overview: Recombinant Human SUMO2 (Accession # NM_006937) , fused with a 6-His tag, was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: X mg/ml (X µM) in 50 mM HEPES pH 8.0, 250 mM NaCl, 1mM DTT, 1mM EDTA. Actual concentration varies with lot number.
• Bio-activity: Recombinant Human His6-Pro-SUMO2 can be conjugated to substrate proteins via the subsequent actions of an SUMO-activating (E1) enzyme, an SUMO-conjugating (E2) enzyme, and an SUMO ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Pro-SUMO2 concentration of 10-50 µM.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SUMO2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: SUMO2
• Synonyms: SUMO2; SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae); SMT3 (suppressor of mif two 3, yeast) homolog 2 , SMT3 suppressor of mif two 3 homolog 2 (yeast) , SMT3H2; small ubiquitin-related modifier 2; SMT3B; sentrin 2; SMT3 homolog 2; ubiquitin-like protein SMT3A; ubiquitin-like protein SMT3B; small ubiquitin-like modifier 2; HSMT3; SUMO3; Smt3A; SMT3H2; MGC117191
• Gene ID: 6613
• mRNA Refseq: NM_001005849
• Protein Refseq: NP_001005849
• MIM: 603042
• UniProt ID: P61956
• Chromosome Location: 17q25