Active Recombinant Human SRC protein, His/GST-tagged

• Cat.No.: SRC-1358H
• Product Overview: Recombinant Human SRC(Met 1-Leu 536) fused with His/GST tag at N-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-536 a.a.
• Description: This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
• Predicted N Terminal: Met
• Form: Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% gly, 0.3mM DTT
• Bio-activity: The specific activity was determined to be 27 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
• Molecular Mass: The recombinant human SRC/GST chimera consists of 773 amino acids and has a calculated molecular mass of 87.7 KDa. It migrates as an approximately 81 KDa band in SDS-PAGE under non-reduced conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Shipping: Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
  Shipment made at ambient temperature may seriously affect the activity of the ordered products.

Gene Information

• Gene Name: SRC SRC proto-oncogene, non-receptor tyrosine kinase [ Homo sapiens ]
• Official Symbol: SRC
• Synonyms: ASV; SRC1; THC6; c-SRC; p60-Src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
• Gene ID: 6714
• mRNA Refseq: NM_005417
• Protein Refseq: NP_005408
• MIM: 190090
• UniProt ID: P12931