Active Recombinant Human SPAM1 / PH20 / Hyaluronidase protein, Tag Free

Source :	CHO
Species :	Human
Tag :	Non
Form :	Liquid in sterile 20mM PB, 300mM NaCl, 0.1mM EDTA, 0.1mM CaCl2, pH6.8.
Bio-activity :	The activity of Human Hyaluronidase is measured by its ability to hydrolyze HA in turbidimetric assay. The specific activity is 53,200 IU/mg. (Unit definition: One unit will cause a change in A600nm of 0.330 per minute at pH 5.7 at 37 ºC.)
Molecular Mass :	The protein has a calculated MW of 51 kDa. The protein migrates as 60 kDa under reducing condition (SDS-PAGE) due to glycosylation.
AA Sequence :	LNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFY
Endotoxin :	< 0.1 EU per μg of the protein as determined by the LAL method.
Purity :	> 95% as determined by SDS-PAGE. > 95% as determined by SEC-HPLC.
Storage :	Store it under sterile conditions at -20 to -80 ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Concentration :	0.5mg/ml.

Gene Name	SPAM1 sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) [ Homo sapiens ]
Official Symbol	SPAM1
Synonyms	SPAM1; HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; MGC26532; hyaluronidase PH-20; hyal-PH20; OTTHUMP00000211901; OTTHUMP00000211902; OTTHUMP00000211952; sperm surface protein PH-20; hyaluronoglucosaminidase PH-20
Gene ID	6677
mRNA Refseq	NM_001174044
Protein Refseq	NP_001167515
MIM	600930
UniProt ID	P38567