Active Recombinant Human SORCS1 Protein, His tagged

• Cat.No.: SORCS1-01H
• Product Overview: Active Recombinant Human SORCS1 Protein(Ser111-Ser1099, Ser231Gly) with a C-terminal 6-His tag was expressed in Mouse myeloma cell line.

Specification

• Description: This gene encodes one family member of vacuolar protein sorting 10 (VPS10) domain-containing receptor proteins. The VPS10 domain name comes from the yeast carboxypeptidase Y sorting receptor Vps10 protein. Members of this gene family are large with many exons but the CDS lengths are usually less than 3700 nt. Very large introns typically separate the exons encoding the VPS10 domain; the remaining exons are separated by much smaller-sized introns. These genes are strongly expressed in the central nervous system. Two of the five family members (sortilin and sortilin-related receptor) are synthesized as preproproteins; it is not yet known if this encoded protein is also a preproprotein. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Source: Mammalian cell
• Species: Human
• Tag: C-6×His
• Protein length: 111-1099 aa, Ser231Gly
• Molecular Mass: 112 kDa
• N-terminal Sequence Analysis: Ser111
• Bio-Activity: Measured by its binding ability in a functional ELISA. Immobilized rhSorCS1 at 1 μg/mL (100 μL/well) can bind rh beta-NGF with an apparent KD < 75 nM.
• Endotoxin: < 0.1 EU/μg by LAL.
• Purity: > 85 % by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature.
• Reference: 1. Hampe, W. et al. (2001) Hum. Genet. 108:529.
  2. Hermey, G. et al. (1999) Biochem. Biophys. Res. Commun. 266:347.
  3. Hermey, G. et al. (2003) J. Biol. Chem. 278:7390.

Gene Information

• Gene Name: SORCS1 sortilin related VPS10 domain containing receptor 1 [ Homo sapiens (human) ]
• Official Symbol: SORCS1
• Synonyms: SORCS1; sortilin-related VPS10 domain containing receptor 1; VPS10 domain-containing receptor SorCS1; sorCS1; VPS10 domain receptor protein SORCS 1; hSorCS; FLJ41758; FLJ43475; FLJ44957
• Gene ID: 114815
• mRNA Refseq: NM_001013031
• Protein Refseq: NP_001013049
• MIM: 606283
• UniProt ID: Q8WY21