Active Recombinant Human SOD1 protein, His-tagged

• Cat.No.: SOD1-1161H
• Product Overview: Recombinant Human SOD1 protein(Ala 2 - Gln 154), fused with His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ala 2 - Gln 154
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Measured by its ability to catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen. One Unit is defined as the amount of SOD that inhibits the xanthine oxidase activity by 50% (IC50 (µg)) under the assay conditions. The specific activity is >1000 U.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 16.8 kDa. The protein migrates as 19-21 kDa under reducing (R) condition (SDS-PAGE).
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >97% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SOD1 superoxide dismutase 1, soluble [ Homo sapiens ]
• Official Symbol: SOD1
• Synonyms: SOD1; superoxide dismutase 1, soluble; ALS, ALS1, amyotrophic lateral sclerosis 1 (adult); superoxide dismutase [Cu-Zn]; IPOA; SOD, soluble; indophenoloxidase A; Cu/Zn superoxide dismutase; superoxide dismutase, cystolic; ALS; SOD; ALS1; hSod1; homodimer
• Gene ID: 6647
• mRNA Refseq: NM_000454
• Protein Refseq: NP_000445
• MIM: 147450
• UniProt ID: P00441