Active Recombinant Human SIRT3, GST-tagged

• Cat.No.: SIRT3-347H
• Product Overview: Recombinant Human SIRT3(amino acids 102- 399), fused with N-terminal GST tag, was expressed in E.coli expression system.

Specification

• Description: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: 40 mM Tris-HCl, pH 8.0,Sirt3 Gel 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
• Bio-activity: 5.8 pmole/min/μg.Assay conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 μM BPS HDAC substrate 1, 500 μM NAD+, and Sirtuin 3. Incubation condition: 30 min at 37ºC. Then, incubated with developer for 15 min at room temperature.
• Molecular Mass: 59 kDa
• Purity: ≥56%
• Stability: >6 months at –80°C

Gene Information

• Gene Name: SIRT3 sirtuin 3 [ Homo sapiens (human) ]
• Official Symbol: SIRT3
• Synonyms: SIRT3; sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae); SIR2L3; NAD-dependent deacetylase sirtuin-3, mitochondrial; EC 3.5.1.-; SIR2-like protein 3; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase 2; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3; sirtuin 3; sirtuin type 3
• Gene ID: 23410
• mRNA Refseq: NM_012239
• Protein Refseq: NP_036371
• MIM: 604481
• UniProt ID: Q9NTG7
• Chromosome Location: 11p15.5
• Pathway: Energy Metabolism; Mitochondrial biogenesis; Organelle biogenesis and maintenance
• Function: NOT NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity (H3-K14 specific); protein binding