Active Recombinant Human SIRT1, His-tagged

• Cat.No.: SIRT1-463H
• Product Overview: Human sirtuin 1 (aa 2-555) is fused at the N-terminus to a His-tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-555 a.a.
• Description: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
• Form: Liquid. 0.2μm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM NaCl.
• Bio-activity: HDAC activity.
• Molecular Mass: ~70kDa (SDS-PAGE)
• Endotoxin: <1EU/μg purified protein (LAL test; Lonza).
• Purity: ≥95% (SDS-PAGE).
• Stability: Working aliquots are stable for up to 3 months when stored at -20°C.
• Storage: Short Term Storage: +4°CLong Term Storage: -20°CHandling Advice: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: SIRT1 sirtuin 1 [ Homo sapiens (human) ]
• Official Symbol: SIRT1
• Synonyms: SIRT1; SIR2L1; sirtuin 1; NAD-dependent protein deacetylase sirtuin-1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; NAD-dependent deacetylase sirtuin-1
• Gene ID: 23411
• mRNA Refseq: NM_001142498
• Protein Refseq: NP_001135970
• MIM: 604479
• UniProt ID: Q96EB6
• Chromosome Location: 10q21.3
• Pathway: Amphetamine addiction; Androgen receptor signaling pathway; FoxO signaling pathway
• Function: HLH domain binding; NOT NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity