Active Recombinant Human SFRP5 Protein, HA-tagged

• Cat.No.: SFRP5-2621H
• Product Overview: Recombinant Human SFRP5 Protein (Glu30-His317) with an N-terminal HA (YPYDVPDYA) tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Description: Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.
• Source: CHO
• Species: Human
• Tag: HA
• Predicted N Terminal: Tyr (HA), Trp37 & Ser46
• Bio-activity: Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.25-1 μg/mL in the presence of 100 ng/mL Recombinant Mouse Wnt‑3a.
• Molecular Mass: 30-40 kDa, reducing conditions
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Protein length: Glu30-His317

Gene Information

• Gene Name: SFRP5 secreted frizzled related protein 5 [ Homo sapiens (human) ]
• Official Symbol: SFRP5
• Synonyms: SFRP5; secreted frizzled related protein 5; SARP3; secreted frizzled-related protein 5; FRP-1b; SARP-3; frizzled-related protein 1b; sFRP-5; secreted apoptosis related protein 3
• Gene ID: 6425
• mRNA Refseq: NM_003015
• Protein Refseq: NP_003006
• MIM: 604158
• UniProt ID: Q5T4F7

Reviews

05/20/2022

Satisfied customer—great product!

08/02/2019

Impressed with the quality control. This product is a gem in our lab experiments.

12/17/2018

Outstanding lab product!

Q&As

How is SFRP5 expression regulated at the transcriptional and post-transcriptional levels, and what factors influence its expression in different tissues?

SFRP5 expression is regulated by various factors, including transcriptional regulators and microRNAs. Tissue-specific expression is influenced by developmental cues and environmental signals.

Can you elaborate on the structural features of the SFRP5 protein and how they contribute to its interactions with Wnt ligands?

SFRP5 has a cysteine-rich domain that interacts with Wnt ligands, preventing their binding to Frizzled receptors. The structural configuration is crucial for modulating Wnt signaling.

Are there any identified genetic variations or polymorphisms in the SFRP5 gene, and how might they influence individual susceptibility to certain diseases?

Genetic variations in the SFRP5 gene have been identified, and certain polymorphisms are associated with altered susceptibility to diseases such as cancer and metabolic disorders.

How does SFRP5 contribute to tissue regeneration and repair, and what are the implications for regenerative medicine?

SFRP5 plays a role in tissue regeneration by modulating Wnt signaling during repair processes. Understanding its function contributes to the development of regenerative medicine strategies.

What are the implications of altered SFRP5 expression in diseases, particularly in conditions where dysregulated Wnt signaling is implicated?

Altered SFRP5 expression is associated with various diseases, including cancer and metabolic disorders, where dysregulated Wnt signaling plays a role in disease progression.