Active Recombinant Full Length Human SERPINF2, His tagged

• Cat.No.: SERPINF2-3159H
• Product Overview: Recombinant Human SERPINF2 (NP_000925.2) (Met 1-Lys 491), fused with a C-terminal polyhistidine tag, was produced in Human Cell.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate,Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec). The IC50 value is < 0.5 nM .
• Molecular Mass: The recombinant human SerpinF2 consists of 475 amino acids after removal of the signal peptide and predicts a molecular mass of 53.2 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rhSerpinF2 is approximately 70 kDa due to glycosylation.
• Protein length: Met 1-Lys 491
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: SERPINF2 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2 [ Homo sapiens ]
• Official Symbol: SERPINF2
• Synonyms: SERPINF2; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2; PLI, serine (or cysteine) proteinase inhibitor, clade F (alpha 2 antiplasmin, pigment epithelium derived factor), member 2; alpha-2-antiplasmin; A2AP; AAP; alpha 2 antiplasmin; ALPHA 2 PI; alpha 2 plasmin inhibitor; API; serpin F2; alpha-2-AP; alpha-2-plasmin inhibitor; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2; PLI; ALPHA-2-PI
• Gene ID: 5345
• mRNA Refseq: NM_000934
• Protein Refseq: NP_000925
• MIM: 613168
• UniProt ID: P08697