Active Recombinant Human SERPINB3 Protein, His-tagged

• Cat.No.: SERPINB3-506H
• Product Overview: Recombinant Human SERPINB3 (Asn2-Pro390) protein with an N-terminal Met and 6-His tag was expressed in Sf 21.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: His
• Protein Length: 2-390 a.a.
• Description: Serpin B3, also known as squamous cell carcinoma antigen-1 (SCCA-1), is a member of the serpin superfamily of serine protease inhibitors (1). Serpins primarily inhibit serine proteases and some are known to inhibit caspases and papain-like cysteine proteases (1). Serpin B3 belongs to the subgroup ovalbumin-related serpins which are involved in the regulation of apoptosis, inflammation, angiogenesis and embryogenesis (2). Serpin B3 was initially isolated from human cervical squamous cell tissue. It is a tumor marker for squamous cell carcinomas in various areas including the cervix, head, neck and lung (3). Unlike typical serpins, Serpin B3 shows inhibitory activity against papain-like lysosomal cysteine proteases Cathepsin K, L, and S, while lacking inhibitory activity against serine proteases (4).
• Bio-activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC. The IC50 is <5 nM.
• Molecular Mass: 45 kDa
• N-terminal Sequence Analysis: Met
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 3 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.

Gene Information

• Gene Name: SERPINB3 serpin family B member 3 [ Homo sapiens (human) ]
• Official Symbol: SERPINB3
• Synonyms: SERPINB3; serpin family B member 3; HsT1196; Protein T4-A; SCC; SCCA; SCCA1; SCCA1SCCA-1; SCCA-PD; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3; Serpin B3; serpin peptidase inhibitor, clade B (ovalbumin), member 3; Squamous cell carcinoma antigen 1; T4-A; SSCA1; SCCA-1; serpin B3; protein T4-A; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3; serpin peptidase inhibitor, clade B (ovalbumin), member 3; squamous cell carcinoma antigen 1
• Gene ID: 6317
• mRNA Refseq: NM_006919
• Protein Refseq: NP_008850
• MIM: 600517
• UniProt ID: P29508