Active Recombinant Human PTPN11 Protein, His-tagged

• Cat.No.: PTPN11-519H
• Product Overview: Recombinant Human PTPN11 (Accession # NP_002825) Ser2-Arg593, fused with an N-terminal 6-His tag, was produced in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ser2-Arg593
• Predicted N Terminal: His
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, Glycerol and DTT.
• Bio-activity: Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site.The specific activity is >1.2 µmol/min/mg, measured under the described conditions.
• Molecular Mass: Recombinant Human PTPN11, His-tagged has a calculated MW of 69 kDa. In SDS-PAGE migrates as 63-70 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: PTPN11 protein tyrosine phosphatase, non-receptor type 11 [ Homo sapiens ]
• Official Symbol: PTPN11
• Synonyms: PTPN11; protein tyrosine phosphatase, non-receptor type 11; Noonan syndrome 1 , NS1; tyrosine-protein phosphatase non-receptor type 11; BPTP3; PTP2C; SH PTP2; SHP 2; SHP2; PTP-2C; protein tyrosine phosphatase-2; protein-tyrosine phosphatase 1D; protein-tyrosine phosphatase 2C; CFC; NS1; PTP-1D; SH-PTP2; SH-PTP3; MGC14433
• Gene ID: 5781
• mRNA Refseq: NM_002834
• Protein Refseq: NP_002825
• MIM: 176876
• UniProt ID: Q06124
• Chromosome Location: 12q24.1