Active Recombinant Human PRMT7, FLAG-tagged

• Cat.No.: PRMT7-68H
• Product Overview: Recombinant Human PRMT7 (accession number NP_061896.1) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 80.1 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: Flag
• Description: PRMT7 (Protein Arginine Methyltransferase 7) is a type I arginine methyltransferase. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT7 specifically mediates the symmetrical dimethylation of histone H4 at Arg3 to form H4R3me2s. PRMT7 plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruitment of DNA methyltransferases at these sites. PRMT7 may also play a role in embryonic stem cell (ESC) pluripotency. PRMT7 is also able to mediate arginine methylation of histone H2A and myelin basic protein (MBP) in vitro. However, the biological relevance of such results is unclear.
• Form: 25 mM HEPES, pH 7.5, 150 mM NaCl and 5% glycerol.
• Bio-activity: Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3). Specifically mediates the symmetric dimethylation of histone H4 "Arg-3" to form H4R3me2s.
• Applications: Enzyme kinetics, inhibitor screening, and selectivity profiling.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 0.8 mg/ml

Gene Information

• Gene Name: PRMT7 protein arginine methyltransferase 7 [ Homo sapiens ]
• Official Symbol: PRMT7
• Synonyms: PRMT7; protein arginine methyltransferase 7; protein arginine N-methyltransferase 7; FLJ10640; KIAA1933; histone-arginine N-methyltransferase PRMT7; myelin basic protein-arginine N-methyltransferase; [Myelin basic protein]-arginine N-methyltransferase PRMT7
• Gene ID: 54496
• mRNA Refseq: NM_001184824
• Protein Refseq: NP_001171753
• MIM: 610087
• UniProt ID: Q9NVM4
• Chromosome Location: 16q22.1
• Function: S-adenosylmethionine-dependent methyltransferase activity; [myelin basic protein]-arginine N-methyltransferase activity; histone binding; histone methyltransferase activity (H4-R3 specific); histone-arginine N-methyltransferase activity; protein methyltransferase activity; protein-arginine omega-N monomethyltransferase activity; protein-arginine omega-N symmetric methyltransferase activity; protein-arginine omega-N symmetric methyltransferase activity; ribonucleoprotein complex binding; transferase activity