Active Recombinant Human PRELP Protein, His tagged

• Cat.No.: PRELP-20HFL
• Product Overview: Active Recombinant Human PRELP Protein (Gln21-Ile382) with an N-terminal 6-His tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Description: The protein encoded by this gene is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage. Alternatively spliced transcript variants encoding the same protein have been observed.
• Source: CHO
• Species: Human
• Tag: N-6×His
• Protein length: 21-382 aa
• Molecular Mass: 42.5 kDa
• N-terminal Sequence Analysis: His
• Bio-Activity: Measured by its binding ability in a functional ELISA. Recombinant Human PRELP binds Recombinant Human Aggrecan with an ED50 of 10.0-120 ng/mL.
• Endotoxin: < 0.1 EU/μg by LAL.
• Purity: > 95 % by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after opening.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in PBS.
• Shipping: The product is shipped with dry ice or equivalent.
• Reference: 1. Bengtsson, E. et al. (1995) J. Biol. Chem. 270:25639.
  2. Merline, R. et al. (2009) J. Cell Commun. Signal. 3:323.
  3. McEwan, P.A. et al. (2006) J. Struct. Biol. 155:294.

Gene Information

• Gene Name: PRELP proline and arginine rich end leucine rich repeat protein [ Homo sapiens (human) ]
• Official Symbol: PRELP
• Synonyms: PRELP; proline/arginine-rich end leucine-rich repeat protein; proline arginine rich end leucine rich repeat protein; prolargin; prolargin proteoglycan; SLRR2A; proline-arginine-rich end leucine-rich repeat protein; 55 kDa leucine-rich repeat protein of articular cartilage; MST161; MSTP161; MGC45323
• Gene ID: 5549
• mRNA Refseq: NM_002725
• Protein Refseq: NP_002716
• MIM: 601914
• UniProt ID: P51888