Active Recombinant Human PRCP, His-tagged

• Cat.No.: PRCP-23H
• Product Overview: Recombinant Human PRCP (Met1-His496), fused with a C-terminal 6-His tag, was expressed in Mouse NSO Cells.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 1-496 a.a.
• Description: The protein encoded by this gene is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Predicted N Terminal: Leu22
• Form: Supplied as a 0.2 µm filtered solution in MES and NaCl.
• Bio-activity: Measured by its ability to cleave the synthetic peptide substrate N-benzyloxycarbonyl-Pro-Ala (Z-Pro-Ala).
• Molecular Mass: Predicted Molecular Mass: 54 kDaSDS-PAGE: 60-70 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 °C as supplied.3 months, -20 to -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: PRCP prolylcarboxypeptidase (angiotensinase C) [ Homo sapiens ]
• Official Symbol: PRCP
• Synonyms: PRCP; prolylcarboxypeptidase (angiotensinase C); lysosomal Pro-X carboxypeptidase; HUMPCP; PCP; angiotensinase C; proline carboxypeptidase; lysosomal carboxypeptidase C; prolylcarboxypeptidase isoform 1 preproprotein; MGC2202
• Gene ID: 5547
• mRNA Refseq: NM_005040
• Protein Refseq: NP_005031
• MIM: 176785
• UniProt ID: P42785
• Chromosome Location: 11q14
• Pathway: Formation of Fibrin Clot (Clotting Cascade); Hemostasis; Protein digestion and absorption; Intrinsic Pathway
• Function: protein binding; serine-type carboxypeptidase activity