Active Recombinant Human PLG Protein, Fc Chimera

• Cat.No.: PLG-1107H
• Product Overview: Recombinant Human PLG (Accession # P00747), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Met-Asp-Pro100
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of THP-1 human acute monocytic leukemia cells. Chavakis, T. et al. (2005) Blood 10^5:1036.The ED50 for this effect is typically 0.5-2.5 µg/mL.
• Molecular Mass: Recombinant Human PLG, Fc Chimera has a calculated MW of 67.7 kDa (monomer). In SDS-PAGE migrates as 75-85 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE with silver staining, under reducing conditions.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: PLG plasminogen [ Homo sapiens ]
• Official Symbol: PLG
• Synonyms: PLG; plasminogen; plasmin; DKFZp779M0222
• Gene ID: 5340
• mRNA Refseq: NM_001168338
• Protein Refseq: NP_001161810
• MIM: 173350
• UniProt ID: P00747
• Chromosome Location: 6q26