Active Recombinant Human Phospho-UBB protein

• Cat.No.: UBB-01HP
• Product Overview: E. coli-derived human Ubiquitin protein Phosphorylated on Ser 65.

Specification

• Description: This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Form: X mg/ml (X μM) in 10 mM HEPES pH 7.5
• Bio-activity: Reaction conditions will need to be optimized for each specific application.
• Molecular Mass: 8.6 kDa
• Purity: >98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. LC/MS analysis (AQUA) indicates a purity of 95.7%, with 4.3% unmodified Ubiquitin
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 3 months, -20 to -70 centigrade under sterile conditions after opening.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: UBB ubiquitin B [ Homo sapiens (human) ]
• Official Symbol: UBB
• Synonyms: RPS27A; UBA52; UBB; UBB ubiquitin B; UBC; Ubiquitin
• Gene ID: 7314
• mRNA Refseq: NM_018955
• Protein Refseq: NP_061828
• MIM: 191339
• UniProt ID: P0CG47