Active Recombinant Human PECAM1 protein, hFc-tagged

• Cat.No.: PECAM1-014H
• Product Overview: Recombinant Human PECAM1 protein(EAW94208.1)(Met1-Lys601), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 × 10E4 cells/well are added to sCD31-Fc coated plates (5 μg/mL and 100 μL/well) in the presence of 10 μg/ml PHA approximately >60% will adhere after 30 minutes at 37℃.
• Molecular Mass: The recombinant human CD31/Fc is a disulfide-linked homodimer.The reduced monomer consists of 812 amino acids and predicts a molecular mass of 91.2 kDa. As a result of glycosylation, the rhCD31/Fc monomer migrates as approximately 130-140 kDa band in SDS-PAGE under reducing conditions.
• Protein length: Met1-Lys601
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PECAM1 platelet/endothelial cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: PECAM1
• Synonyms: PECAM1; platelet/endothelial cell adhesion molecule 1; platelet/endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule; CD31; CD31 antigen; PECA1; GPIIA; PECAM-1; endoCAM; CD31/EndoCAM; FLJ34100; FLJ58394
• Gene ID: 5175
• mRNA Refseq: NM_000442
• Protein Refseq: NP_000433
• MIM: 173445
• UniProt ID: P16284