Active Recombinant Human PDCD1 protein, mFc-Avi-tagged, Biotinylated

• Cat.No.: PDCD1-0612H
• Product Overview: Biotinylated Recombinant Human PDCD1 protein(Leu25-Gln167), fused with mFc and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&mFc
• Protein Length: 25-167 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human PD-L1, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Mouse IgG2a Fc,Avitag with a linear range of 5-156 ng/mL. Immobilized Nivolumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Mouse IgG2a Fc,Avitag with a linear range of 0.2-5 ng/mL. Immobilized Human PD-L2, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Mouse IgG2a Fc,Avitag with a linear range of 1-78 ng/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 44.5 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu25-Gln167
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Homo sapiens ]
• Official Symbol: PDCD1
• Synonyms: PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l
• Gene ID: 5133
• mRNA Refseq: NM_005018
• Protein Refseq: NP_005009
• MIM: 600244
• UniProt ID: Q15116