Active Recombinant Human PDCD1 Protein, LIgG2b Fc-tagged, low endotoxin

• Cat.No.: PDCD1-361H
• Product Overview: Recombinant Human PDCD1 Protein, LIgG2b Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1). Predicted N-terminus: Leu 25. This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 43.8 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Source: HEK293
• Species: Human
• Tag: lIgG2b
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human PD-L1, Mouse IgG1 Fc Tag (Cat. No. PD1-H52A3) at 0.5 μg/mL (100 μL/well) can bind Human PD-1, Llama IgG2b Fc Tag, low endotoxin (Cat. No. PD1-H5259) with a linear range of 20-313 ng/mL (QC tested).
  Immobilized Human PD-1, Llama IgG2b Fc Tag, low endotoxin (Cat. No. PD1-H5259) at 2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.1-2 ng/mL (Routinely tested).
• Molecular Mass: 43.8 kDa
• Protein length: Leu 25 - Gln 167
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Homo sapiens ]
• Official Symbol: PDCD1
• Synonyms: PDCD1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l
• Gene ID: 5133
• mRNA Refseq: NM_005018
• Protein Refseq: NP_005009
• MIM: 600244
• UniProt ID: Q15116