Active Recombinant Human PDCD1 Protein, Fc-His-tagged, PE-Labeled

• Cat.No.: PDCD1-4688H
• Product Overview: PE-Labeled Recombinant Human Protein(Leu 25 - Gln 167), fused with Fc-His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His&Fc
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Flow Cytometry assay shows that PE-Labeled Human PD-1, Fc,His Tag can bind to 293T cells overexpressing human PD-L1. 1 μL stock solution per million cells.FACS analysis shows that the binding of PE-Labeled Human PD-1, Fc,His Tag to 293T overexpressing PD-L1 was inhibited by increasing concentration of neutralizing anti-human PD-L1 antibody. The IC50 is 0.29 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag.The protein has a calculated MW of 44.7 kDa.
• Protein length: Leu 25 - Gln 167
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Homo sapiens ]
• Official Symbol: PDCD1
• Synonyms: PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l
• Gene ID: 5133
• mRNA Refseq: NM_005018
• Protein Refseq: NP_005009
• MIM: 600244
• UniProt ID: Q15116