Active Recombinant Human PDCD1, no tag

• Cat.No.: PDCD1-172H
• Product Overview: Recombinant Human PDCD1(Leu 25 - Leu 288), fused with no tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: Leu 25 - Leu 288
• Description: Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
• Predicted N Terminal: Leu 25
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Bio-activity: Immobilized rh full length PDCD1 /PD1 at 2 μg/ml ( 100 μl/well ) can bind Human PD-L1 /B7-H1 Fc Chimera with a linear range of 20 - 200 ng/ml.
• Molecular Mass: DTT-reduced Protein migrates as 29-70 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the rh PDCD1 /PD1 (full length) by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Storage: No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
• Warning: Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Homo sapiens ]
• Official Symbol: PDCD1
• Synonyms: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; programmed cell death protein 1; protein PD-1; systemic lupus erythematosus susceptibility 2
• Gene ID: 5133
• mRNA Refseq: NM_005018
• Protein Refseq: NP_005009
• MIM: 600244
• UniProt ID: Q15116
• Chromosome Location: 2q37.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem
• Function: protein binding; signal transducer activity