Active Recombinant Human PADI4, His-tagged

• Cat.No.: PADI4-380H
• Product Overview: Recombinant Human PADI4 (Ala2-Pro663) fused with an N-terminal 11 kDa fusion protein, a thrombin cleavage linker and 6­His tag at C­terminal, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-663 a.a.
• Description: This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response.
• Predicted N Terminal: Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments
• Formulation: Supplied as a 0.2 µm filtered solution in Sodium Acetate, NaCl, DTT, EDTA and Glycerol.
• Bio-activity: Measured by its ability to catalyze the deimination of benzoyl-arginine ethyl ester. The specific activity is >1000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 87 kDa SDS-PAGE: 75-85 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: PADI4 peptidyl arginine deiminase, type IV [ Homo sapiens (human) ]
• Official Symbol: PADI4
• Synonyms: PADI4; PAD; PAD4; PDI4; PDI5; PADI5; peptidyl arginine deiminase, type IV; protein-arginine deiminase type-4; HL-60 PAD; PADI-H protein; protein-arginine deiminase type IV; peptidyl arginine deiminase, type V; EC 3.5.3.15
• Gene ID: 23569
• mRNA Refseq: NM_012387
• Protein Refseq: NP_036519
• MIM: 605347
• UniProt ID: Q9UM07
• Chromosome Location: 1p36.13
• Pathway: protein citrullination
• Function: calcium ion binding; protein-arginine deiminase activity