Active Recombinant Human NTRK3 protein(Met1-Asp428), His&hFc-tagged

• Cat.No.: NTRK3-159H
• Product Overview: Recombinant Human TrkC (NP_001007157.1)(Met1-Asp428) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His&hFc
• Protein length: Met1-Asp428
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized human TrKc-Fch at 10 μg/ml (100 μl/well) can bind biotinylated human NT3, The EC50 of biotinylated human NT3 is 23.4-54.6 ng/ml.
• Molecular Mass: The recombinant human TrkC/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. It consists of 644 amino acids and has a calculated molecular mass of 72.5 kDa. As a result of glycosylation, rh TrkC/Fc monomer migRates as an approximately 110-120 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NTRK3 neurotrophic tyrosine kinase, receptor, type 3 [ Homo sapiens ]
• Official Symbol: NTRK3
• Synonyms: NTRK3; neurotrophic tyrosine kinase, receptor, type 3; NT-3 growth factor receptor; TRKC; GP145-TrkC; ETV6-NTRK3 fusion; tyrosine kinase receptor C; ETS related protein-neurotrophic receptor tyrosine kinase fusion protein; gp145(trkC)
• Gene ID: 4916
• mRNA Refseq: NM_001007156
• Protein Refseq: NP_001007157
• MIM: 191316
• UniProt ID: Q16288