Active Recombinant Human NTN1 protein, His-tagged

• Cat.No.: NTN1-0575H
• Product Overview: Recombinant Human NTN1 protein(Val22-Ala604), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 22-604 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with NaCl. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Netrin-1, His Tag at 5 μg/mL (100 μL/well) can bind Human Netrin receptor DCC, Fc Tag with a linear range of 1-10 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 67.5 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Val22-Ala604
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NTN1 netrin 1 [ Homo sapiens ]
• Official Symbol: NTN1
• Synonyms: NTN1; netrin 1; netrin-1; Netrin 1; NTN1L; netrin 1, mouse, homolog of
• Gene ID: 9423
• mRNA Refseq: NM_004822
• Protein Refseq: NP_004813
• MIM: 601614
• UniProt ID: O95631