Active Recombinant Human NT5E, His-tagged

• Cat.No.: NT5E-343H
• Product Overview: Recombinant Human NT5E (Accession # AAH65937) Trp27-Lys547, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Human
• Tag: His
• Predicted N Terminal: Trp27
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
• Bio-activity: Measured by its ability to hydrolyze the 5"-phosphate group from the substrate adenosine-5"-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit .The specific activity is >15,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human NT5E, His-tagged has a calculated MW of 59 kDa. In SDS-PAGE migrates as 61-62 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Protein length: 27-547 a.a.

Gene Information

• Gene Name: NT5E 5-nucleotidase, ecto (CD73) [ Homo sapiens ]
• Official Symbol: NT5E
• Synonyms: NT5E; 5-nucleotidase, ecto (CD73); 5 nucleotidase (CD73) , NT5; 5-nucleotidase; CD73; eN; eNT; 5-NT; ecto-5-nucleotidase; Purine 5-Prime-Nucleotidase; NT; NT5; NTE; E5NT
• Gene ID: 4907
• mRNA Refseq: NM_001204813
• Protein Refseq: NP_001191742
• MIM: 129190
• UniProt ID: P21589
• Chromosome Location: 6q14-q21