Active Recombinant Human NRP1 protein, mFc-tagged

• Cat.No.: NRP1-3347H
• Product Overview: Recombinant Human NRP1 protein(Phe 22 - Lys 644), fused with mFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: mFc
• Protein Length: Phe 22 - Lys 644
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human VEGF165, His Tag at 2 μg/mL (100 μL/well) can bind Human Neuropilin-1, Mouse IgG2a Fc Tag with a linear range of 0.2-5 ng/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminusThe protein has a calculated MW of 96.7 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NRP1 neuropilin 1 [ Homo sapiens ]
• Official Symbol: NRP1
• Synonyms: NRP1; neuropilin 1; neuropilin-1; CD304; NRP; VEGF165R; transmembrane receptor; vascular endothelial cell growth factor 165 receptor; NP1; BDCA4; DKFZp781F1414; DKFZp686A03134
• Gene ID: 8829
• mRNA Refseq: NM_001024628
• Protein Refseq: NP_001019799
• MIM: 602069
• UniProt ID: O14786