Active Recombinant Human NPR3 Protein (Thr24-Glu481), C-Fc tagged

• Cat.No.: NPR3-12H
• Product Overview: Recombinant Human NPRC/NPR3 Protein (Thr24-Glu481, Accession # P17342.2) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Thr24-Glu481
• Description: This gene encodes one of three natriuretic peptide receptors. Natriutetic peptides are small peptides which regulate blood volume and pressure, pulmonary hypertension, and cardiac function as well as some metabolic and growth processes. The product of this gene encodes a natriuretic peptide receptor responsible for clearing circulating and extracellular natriuretic peptides through endocytosis of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene.
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human NPRC/NPR3 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Osteocrin that produces 50% of the optimal binding response is 2-20 ng/mL
• Molecular Mass: Predicted Molecular Mass: 77 kDa & 75 kDa
  SDS-PAGE: 78-92 kDa
• N-terminal Sequence Analysis: Thr24 & Glu46
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: NPR3 natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C) [ Homo sapiens (human) ]
• Official Symbol: NPR3
• Synonyms: NPR3; natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C); ANPRC, C5orf23, chromosome 5 open reading frame 23 , NPRC; atrial natriuretic peptide receptor 3; FLJ14054; GUCY2B; atrial natriuretic peptide receptor type C; atrial natriuretic peptide clearance receptor; NPRC; ANP-C; ANPRC; NPR-C; ANPR-C; C5orf23; MGC22189
• Gene ID: 4883
• mRNA Refseq: NM_000908
• Protein Refseq: NP_000899
• MIM: 108962
• UniProt ID: P17342