Active Recombinant Human NGF Protein, Animal Free

• Cat.No.: NGF-41H
• Product Overview: Recombinant Human NGF Protein without tag was expressed in Human cells.
  Animal Free and Xeno Free

Specification

• Species: Human
• Source: Human Cells
• Description: Nerve growth factors (NGFs) is composed of three noncovalently linked subunits, α, β, and γ, while the β subunit, called NGF-β exhibits all the biological activities ascribed to NGF. NGF-β is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF-β is a well characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF-β can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF-β has also been shown to have biological effects on non-neuronal tissues.
• Bio-activity: The activity was measured by its ability to stimulate the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
• Molecular Mass: 13 kDa homotrimer
• Endotoxin: <0.5 EU/ μg
• Purity: High Purity, >95% by SDS-PAGE
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -80 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS without carrier protein
• Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Gene Information

• Gene Name: NGF nerve growth factor (beta polypeptide) [ Homo sapiens (human) ]
• Official Symbol: NGF
• Synonyms: NGF; nerve growth factor (beta polypeptide); NGFB; beta-nerve growth factor; nerve growth factor, beta subunit; HSAN5; Beta-NGF; MGC161426; MGC161428
• Gene ID: 4803
• mRNA Refseq: NM_002506
• Protein Refseq: NP_002497
• MIM: 162030
• UniProt ID: P01138