Active Recombinant Human NGF

Source :	Mammalian cells
Species :	Human
Predicted N Terminal :	Ser122
Form :	Lyophilized from a 0.2 µm filtered solution in Acetic Acid with BSA as a carrier protein.
Bio-activity :	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.The ED50 for this effect is 0.2-2 ng/mL.
Molecular Mass :	Recombinant Human NGF has a calculated MW of 13.5 kDa (monomer). In SDS-PAGE migrates as 13 kDa, reducing conditions.
Purity :	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage :	Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution :	Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Tag :	Non
Protein length :	1-241 a.a.

Gene Name	NGF nerve growth factor (beta polypeptide) [ Homo sapiens ]
Official Symbol	NGF
Synonyms	NGF; nerve growth factor (beta polypeptide); NGFB; beta-nerve growth factor; nerve growth factor, beta subunit; HSAN5; Beta-NGF; MGC161426; MGC161428;
Gene ID	4803
mRNA Refseq	NM_002506
Protein Refseq	NP_002497
MIM	162030
UniProt ID	P01138
Chromosome Location	1p13.1