Active Recombinant Human MUC16 protein, His-tagged

• Cat.No.: MUC16-0273H
• Product Overview: Recombinant Human MUC16 protein(Pro13810-Pro14451), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Immobilized Recombinant Human MSLN / Mesothelin Protein at 2 μg/ml (100 μl/well) can bind Recombinant Human CA125 Protein (ECD, His Tag), the EC50 is 6-18 ng/mL.
• Molecular Mass: The recombinant human MUC16 consists of 653 amino acids and predicts a molecular mass of 73.27 kDa. It migrates as an approximately 101.25 kDa band in SDS-PAGE under reducing conditions.
• Proteinlength: Pro13810-Pro14451
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: Pro13810-Pro14451

Gene Information

• Gene Name: MUC16 mucin 16, cell surface associated [ Homo sapiens ]
• Official Symbol: MUC16
• Synonyms: MUC16; mucin 16, cell surface associated; mucin-16; CA125; FLJ14303; CA-125; MUC-16; CA125 ovarian cancer antigen; ovarian carcinoma antigen CA125; ovarian cancer-related tumor marker CA125
• Gene ID: 94025
• mRNA Refseq: NM_024690
• Protein Refseq: NP_078966
• MIM: 606154
• UniProt ID: Q8WXI7

Reviews

07/28/2020

Critical for decision-making. Supports our research endeavors.

05/24/2018

Expert service quality. Valuable for research integrity.

06/05/2017

Dependable partner in experiments. Consistent and trustworthy.

Q&As

How does MUC16 interact with other components of the epithelial barrier and immune system?

MUC16 works with other mucins and immune components to maintain epithelial barrier function and immune responses.

How does MUC16 contribute to the formation of the mucosal barrier?

It aids in forming the mucosal barrier, protecting against pathogens and mechanical damage.

What potential therapeutic applications arise from targeting MUC16 in cancer treatment?

Targeting MUC16 in cancer therapy, especially in ovarian cancer, could be effective in inhibiting tumor growth and spread.

What is the impact of altered MUC16 activity on inflammatory and infectious diseases?

Altered MUC16 activity can impact the body's defense against inflammation and infection.

What is the primary role of MUC16 in epithelial cell function?

MUC16, a mucin protein, is essential for maintaining the protective mucous layer on epithelial surfaces.

What role does MUC16 play in cancer, particularly ovarian cancer?

MUC16 is associated with cancer progression, particularly in ovarian cancer, where it may facilitate tumor growth and metastasis.

How do genetic variations in MUC16 affect its function in mucosal protection and cancer development?

Genetic variations in MUC16 can lead to differences in mucosal integrity and potentially influence cancer susceptibility.