Active Recombinant Human/Mouse/Rat INHBA Protein (Gly311-Ser426), GMP Grade

• Cat.No.: INHBA-09HG
• Product Overview: GMP recombinant human/Mouse/Rat Activin A protein (Gly311-Ser426) without tag was expressed in Chinese Hamster Ovary cell line and manufactured and tested under cGMP guidelines.

Specification

• Species: Human/Mouse/Rat
• Source: CHO
• Protein Length: Gly311-Ser426
• Description: This gene encodes a member of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate a subunit of the dimeric activin and inhibin protein complexes. These complexes activate and inhibit, respectively, follicle stimulating hormone secretion from the pituitary gland. The encoded protein also plays a role in eye, tooth and testis development. Elevated expression of this gene may be associated with cancer cachexia in human patients.
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 0.2-1.2 ng/mL.
  The specific activity of Recombinant Human/Mouse/Rat Activin A is >1000 units/mg, which is calibrated against the human Activin A WHO reference standard.
• Molecular Mass: Predicted Molecular Mass: 13 kDa (monomer)
  SDS-PAGE: 14 kDa, reducing conditions; 24 kDa, non-reducing conditions
• N-terminal Sequence Analysis: Gly311-Leu-Glu-(Cys)-Asp-Gly-Lys-Val-Asn-Ile
• Endotoxin: <0.10 EU/ μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Reconstitution: Reconstitute at 100-500 μg/mL in sterile 4 mM HCl.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: INHBA inhibin, beta A [ Homo sapiens ]
• Official Symbol: INHBA
• Synonyms: INHBA; inhibin, beta A; inhibin, beta A (activin A, activin AB alpha polypeptide); inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; FSH-releasing protein; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; EDF; FRP
• Gene ID: 3624
• mRNA Refseq: NM_002192
• Protein Refseq: NP_002183
• MIM: 147290
• UniProt ID: P08476