Active Recombinant Human MMP7

• Cat.No.: MMP7-40H
• Product Overview: Recombinant Human Matrix Metalloproteinase-7 produced inE.Coliis a single, non-glycosylated, polypeptide chain containing having a molecular mass of 19.13 kDa. The MMP-7 is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28 kDa proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18 kDa active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas.
• Physical Appearance: Sterile clear liquid solution.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein (0.83 mg/ml) contains the following additives 10 mM HEPES (pH 7.4), 5 mM CaCl2 and 150 mM NaCl.
• Biological Activity: The specific activity was found to be 3100 IU/mg.
• Unit Definition: One unit is defined as the digestion of 1 µg Azocoll/min at 37°C.
• Recommended Use: Western Blotting (1:1K-5K for neat serum and 1-10 ug/ml for affinity pure antibody using ECL technique). ELISA: Control peptide can be used to coat ELISA plates at 1 ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1 ug/ml for affinity pure). Histochemistry & Immunofluorescence: Not tested.
• Stability: Matrilysin although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: MMP7 matrix metallopeptidase 7 (matrilysin, uterine) [ Homo sapiens ]
• Synonyms: MMP7; matrix metallopeptidase 7 (matrilysin, uterine); MMP-7; MPSL1; PUMP-1; matrix metalloproteinase 7; matrin; uterine matrilysin; EC 3.4.24.23; Matrix metalloproteinase-7; Pump-1 protease; Uterine metalloproteinase; PUMP1; Matrin
• Gene ID: 4316
• mRNA Refseq: NM_002423
• Protein Refseq: NP_002414
• MIM: 178990
• UniProt ID: P09237
• Chromosome Location: 11q21-q22
• Pathway: Wnt signaling pathway
• Function: calcium ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding