Active Recombinant Human MMP14, His-tagged

• Cat.No.: MMP14-161H
• Product Overview: Recombinant human MMP14 containing 6*his tag at C­terminalwas expressed in mouse myeloma cell, 60 kD (518aa).

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Description: Proteinsof the matrix metalloproteinase (MMP) family are involved in the breakdown ofextracellular matrix in normal physiological processes, such as embryonicdevelopment, reproduction, and tissue remodeling, as well as in diseaseprocesses, such as arthritis and metastasis. Most MMP's are secreted asinactive proproteins which are activated when cleaved by extracellularproteinases. However, the protein encoded by this gene is a member of themembrane-type MMP (MT-MMP) subfamily; each member of this subfamily containsa potential transmembrane domain suggesting that these proteins are expressedat the cell surface rather than secreted. This protein activates MMP2protein, and this activity may be involved in tumor invasion.
• N­terminalSequenceAnalysis: Ala21
• Activity: Measured by its ability to cleave a fluorogenic peptide substrate Mca­KPLGL­Dpa­AR­NH2.The specific activity is >500 pmol/min/μg.
• EndotoxinLevel: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >80%, as determined by SDS­PAGE.
• Formulation: Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl, CaCl2, Glycer-ol.
• Stability&Storage: Use amanual defrost freezer and avoid repeated freeze­thaw cycles. 6 months fromdate of receipt, ­70 °C as supplied. 3 months, ­70 °C under sterileconditions after opening.
• OfficialSymbol: MMP14

Gene Information

• Gene Name: MMP14 matrix metallopeptidase 14(membrane-inserted) [ Homo sapiens ]
• Synonyms: MMP14; matrixmetallopeptidase 14 (membrane-inserted); 1; MMP-X1; MT-MMP; MT1MMP; MTMMP1;MT1-MMP; MT-MMP 1; matrix metalloproteinase-14; membrane type 1metalloprotease; membrane-type matrix metalloproteinase 1; membrane-type-1matrix metalloproteinase; MMP-14; EC 3.4.24.80; OTTHUMP00000027917; EC 3.4.24
• Gene ID: 4323
• mRNA Refseq: NM_004995
• Protein Refseq: NP_004986
• MIM: 600754
• UniProt ID: P50281
• Chromosome Location: 14q11-q12
• Pathway: Activation of Matrix Metalloproteinases; Extracellularmatrix organization; GnRH signaling pathway
• Function: calcium ion binding; integrin binding; metalloendopeptidaseactivity